Introduction to biotechnology, substrates for cell growth, substratew as carbon and nitrogen sources, petrochemicals as substrates for biotechnological products. Mutagenesis, mutants isolation, Molecular and chemical basis of mutagenesis, DNA damaging, kind of mutants, mutagens (chemical, physical), radiations, DNA repair mechanisms. Recombinant DNA technology, lysogenic cycle, bacterial conjugation, transduction, transformation. Genetic engineering (genetic recombination in vitro, recombinant DNA technology, cloning. Restriction enzymes, vectors, plasmids, cosmids, phages, gene bank, gene cloning, complementary DNA, synthetic DNA). PCR, side directed mutagenesis. Industrial fermentations, microorganisms growth kinetics, industrial bioreactors, sterilization, fermentation process, product isolation. Energy from biomass. Chemicals from biomass. Bioremediation of polluted soils and water.
Understand the methods of genetic improvement of microorganism for biotechnological uses Understanding of production biotechnological products.
No specific requirements needed.
Introduction Microorganisms for biotechnological uses Microorganism cultures Genetic improvement of microorganisms Mutagenesis Genetic recombination Protein engineering Downstream processing Sterilization Bioreactors Biotechnological processes.
Level:
Type:
Undergraduate
(A-)
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