Molecular Biology

Heredity, genes and DNA. Heredity and genes. Genes and enzymes. The identification of DNA as the genetic material. Genome structure and organization. Plant cell genome and genome structure of the subcellular organelles (mitochondria, plastids). DNA replication. The expression of the genetic information. Gene-protein relationships. The role of the messenger RNA. The genetic code. RNA viruses and reverse transcription. Transcription, RNA polymerase and transcription factors (classes, structure and functions). Transcriptional, post- transcriptional and translational regulation of gene expression. Protein sorting and its regulation. Signal transduction. Hormones and other signal transducing molecules and their receptors. Signal transduction through membrane-bound and intracellular receptors. Signal transduction pathways. Signal transduction and cytoskeleton. Introduction to the recombinant DNA. Restriction endonucleases. Generation of recombinant DNA molecules. Cloning vectors. Expression of cloned genes. Principles of DNA sequencing. DNA amplification through the use of polymerase chain reaction. Analysis of gene function. Genetic analysis using yeast cells. Introduction of mutation in cloned and cellular genes.

Objectives

Not available

Prerequisites

Not available

Syllabus

Heredity, genes and DNA. Heredity and genes. Genes and enzymes. The identification of DNA as the genetic material. Genome structure and organization. Plant cell genome and genome structure of the subcellular organelles (mitochondria, plastids). DNA replication. The expression of the genetic information. Gene-protein relationships. The role of the messenger RNA. The genetic code. RNA viruses and reverse transcription. Transcription, RNA polymerase and transcription factors (classes, structure and functions). Transcriptional, post- transcriptional and translational regulation of gene expression. Protein sorting and its regulation. Signal transduction. Hormones and other signal transducing molecules and their receptors. Signal transduction through membrane-bound and intracellular receptors. Signal transduction pathways. Signal transduction and cytoskeleton. Introduction to the recombinant DNA. Restriction endonucleases. Generation of recombinant DNA molecules. Cloning vectors. Expression of cloned genes. Principles of DNA sequencing. DNA amplification through the use of polymerase chain reaction. Analysis of gene function. Genetic analysis using yeast cells. Introduction of mutation in cloned and cellular genes.